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cfx96 real time pcr detection system  (Bio-Rad)


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    Bio-Rad cfx96 real time pcr detection system
    Cfx96 Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx96+real+time+pcr+detection+system/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pmc13091180-120-14-19
    Average 96 stars, based on 27901 article reviews
    cfx96 real time pcr detection system - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptomics and 4D-DIA proteomic analysis reveal differences in meat quality between Sichuan white rabbits and New Zealand white rabbits
    Article Snippet: For cDNA synthesis, 1 μg of total RNA from each sample was reverse-transcribed using the HiScript® III RT SuperMix for qPCR (Vazyme, Nanjing, China) following the manufacturer's instructions. .. Quantitative PCR was conducted on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, USA) in a 10 μL reaction volume containing 5 μL SYBR® Green Master Mix (Vazyme), 1 μL cDNA template, 0.5 μL each of forward and reverse primers (10 μM), and 3 μL RNase-free water. ..

    Article Title: The impact of 1-MCP treatment combined with polyethylene film bag packaging on the quality of ‘Shannong Su’ pears during storage
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) was conducted on a CFX96 Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Dietary supplementation with eutectic essential oil improves pork quality and antioxidant capacity in finishing pigs
    Article Snippet: For each sample, 1 μg of total RNA was reverse-transcribed into cDNA using Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR (Yeasen Biotechnology, Shanghai, China), which included a gDNA elimination step. .. RT-qPCR was performed on a CFX96 Real-Time PCR Detection System coupled with a MyCycler Thermal Cycler System (both Bio-Rad, Hercules, CA, USA). ..

    Article Title: Thrombospondin-1 triggers calreticulin expression in human mucoepidermoid carcinoma MC-3 cells via the PERK/CHOP pathway
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse-transcribed using a reverse transcription kit (Thermo Fisher Scientific, Inc.). .. The reverse transcription reaction was performed at 42°C for 15 min, followed by inactivation at 95°C for 5 min. qPCR was subsequently conducted using SYBR Green PCR Master Mix (TaKaRa Biotechnology Co., Ltd.) on a CFX96 Real-Time PCR Detection System (Bio-Rad, USA). ..

    Article Title: Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein response
    Article Snippet: Two micrograms of RNA per sample were reverse transcribed into cDNA using the PrimeScriptTM RT Reagent Kit (RR047A, Takara). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (RK21203, Abclonal) on a CFX96 Real-Time PCR Detection System (BIO-RAD) following the manufacturer's protocol. ..

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: The RT-qPCR was conducted using the AceQ qPCR SYBR Green master mix kit (Nanjing Vazyme Biotech Co., Ltd., Nanjing, China). .. The reactions were conducted in a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA, United States). ..

    Article Title: Caffeic acid phenethyl ester ameliorates high-fat diet-induced muscle textural deterioration in grass carp ( Ctenopharyngodon idellus ) by modulating adipose-muscle crosstalk via myostatin-taz signaling
    Article Snippet: Next, the total RNA was reverse transcribed into cDNA using a PrimeScript RT reagent kit (TaKaRa, Bio Biotechnology Engineering (Dalian) Co., Ltd., Dalian, Liaoning, China). .. The CFX96 Real-Time PCR Detection System (CFX96, Bio-Rad Laboratories, Heracles, CA, USA) was used for qRT-PCR. ..

    Article Title: PEGylated gold nanoparticles regulate metabolic flux in erythrocytes by inducing hemoglobin deoxygenation
    Article Snippet: Subsequently, complementary DNA synthesis was carried out with 3 μg of isolated RNA via the PrimeScript RT reagent kit (Takara). .. For gene expression quantification, amplification reactions were conducted using TB Green Premix Ex Taq II (Takara) on a CFX96 Real-Time PCR Detection System (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Transcriptomics and 4D-DIA proteomic analysis reveal differences in meat quality between Sichuan white rabbits and New Zealand white rabbits
    Article Snippet: For cDNA synthesis, 1 μg of total RNA from each sample was reverse-transcribed using the HiScript® III RT SuperMix for qPCR (Vazyme, Nanjing, China) following the manufacturer's instructions. .. Quantitative PCR was conducted on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, USA) in a 10 μL reaction volume containing 5 μL SYBR® Green Master Mix (Vazyme), 1 μL cDNA template, 0.5 μL each of forward and reverse primers (10 μM), and 3 μL RNase-free water. ..

    Article Title: Thrombospondin-1 triggers calreticulin expression in human mucoepidermoid carcinoma MC-3 cells via the PERK/CHOP pathway
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse-transcribed using a reverse transcription kit (Thermo Fisher Scientific, Inc.). .. The reverse transcription reaction was performed at 42°C for 15 min, followed by inactivation at 95°C for 5 min. qPCR was subsequently conducted using SYBR Green PCR Master Mix (TaKaRa Biotechnology Co., Ltd.) on a CFX96 Real-Time PCR Detection System (Bio-Rad, USA). ..

    Article Title: Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein response
    Article Snippet: Two micrograms of RNA per sample were reverse transcribed into cDNA using the PrimeScriptTM RT Reagent Kit (RR047A, Takara). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (RK21203, Abclonal) on a CFX96 Real-Time PCR Detection System (BIO-RAD) following the manufacturer's protocol. ..

    Quantitative RT-PCR:

    Article Title: Dietary supplementation with eutectic essential oil improves pork quality and antioxidant capacity in finishing pigs
    Article Snippet: For each sample, 1 μg of total RNA was reverse-transcribed into cDNA using Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR (Yeasen Biotechnology, Shanghai, China), which included a gDNA elimination step. .. RT-qPCR was performed on a CFX96 Real-Time PCR Detection System coupled with a MyCycler Thermal Cycler System (both Bio-Rad, Hercules, CA, USA). ..

    Reverse Transcription:

    Article Title: Thrombospondin-1 triggers calreticulin expression in human mucoepidermoid carcinoma MC-3 cells via the PERK/CHOP pathway
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse-transcribed using a reverse transcription kit (Thermo Fisher Scientific, Inc.). .. The reverse transcription reaction was performed at 42°C for 15 min, followed by inactivation at 95°C for 5 min. qPCR was subsequently conducted using SYBR Green PCR Master Mix (TaKaRa Biotechnology Co., Ltd.) on a CFX96 Real-Time PCR Detection System (Bio-Rad, USA). ..

    Polymerase Chain Reaction:

    Article Title: Thrombospondin-1 triggers calreticulin expression in human mucoepidermoid carcinoma MC-3 cells via the PERK/CHOP pathway
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse-transcribed using a reverse transcription kit (Thermo Fisher Scientific, Inc.). .. The reverse transcription reaction was performed at 42°C for 15 min, followed by inactivation at 95°C for 5 min. qPCR was subsequently conducted using SYBR Green PCR Master Mix (TaKaRa Biotechnology Co., Ltd.) on a CFX96 Real-Time PCR Detection System (Bio-Rad, USA). ..

    Gene Expression:

    Article Title: PEGylated gold nanoparticles regulate metabolic flux in erythrocytes by inducing hemoglobin deoxygenation
    Article Snippet: Subsequently, complementary DNA synthesis was carried out with 3 μg of isolated RNA via the PrimeScript RT reagent kit (Takara). .. For gene expression quantification, amplification reactions were conducted using TB Green Premix Ex Taq II (Takara) on a CFX96 Real-Time PCR Detection System (Bio-Rad). ..

    Amplification:

    Article Title: PEGylated gold nanoparticles regulate metabolic flux in erythrocytes by inducing hemoglobin deoxygenation
    Article Snippet: Subsequently, complementary DNA synthesis was carried out with 3 μg of isolated RNA via the PrimeScript RT reagent kit (Takara). .. For gene expression quantification, amplification reactions were conducted using TB Green Premix Ex Taq II (Takara) on a CFX96 Real-Time PCR Detection System (Bio-Rad). ..



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